Mikrobiyoloji Laboratuvarları

The Bacteriology unit of our laboratory is able to carry out microscopic analyses, bacteriological culture analyses and antibiotic sensitivity tests on submitted samples. Pathogens isolated from various clinical samples (sputum, urine, stool, blood, etc.) can be identified using the latest MALDI-TOF MS (matrix-assisted laser desorption-ionization time-of-flight mass spectrometry) method, in addition to other conventional methods. The isolated pathogens are made to undergo a series of antimicrobial sensitivity tests that are in compliance with international standards. To this end, conventional methods such as disk diffusion assays and E-tests are utilized, while antimicrobial sensitivity is determined at the minimum inhibitor concentration level (MIK) using automated bacterial identification systems. Blood, bone marrow and catheter cultures are tested using automated culture systems and are further subjected to the above identification tests and antibiotic sensitivity screenings in the event that pathogens are detected.

The Serology unit of our laboratory is involved in researching microorganisms (bacteria, viruses, fungi, parasites) that play a role in infections or antibodies developed against the body’s indigenous proteins (auto-immune diseases) or the presence of specific microorganism antigens. A wide range of tests are carried out in our laboratory to diagnose infectious diseases; primarily hepatitis, HIV (AIDS) and also rheumatic illnesses. The relevant antigens and antibodies are sought by testing clinical samples taken from patients such as blood, urine, brain and spinal fluid using the latest technologies and are reported in accordance with international diagnostic standards.

Our Mycology unit carries out tests aimed at a laboratory diagnosis of fungal infections and determining the sensitivity of fungal isolates towards antifungal drugs. To this end, direct microscopic imaging, the gold standard for diagnosing such illnesses, and culture methods are used, with fungi produced from such samples being identified in terms of type through the use of conventional methods. Antifungal sensitivity tests, which are instrumental in determining the types of drugs that will be used during treatment, are conducted using recommended known methods that have been developed by international committees. In addition, our Mycology unit employs the Cryptococcus capsule antigen test to support direct microscopic imaging and culturing in order to test for infections that develop as a result of this fungus.

Our Parasitology unit conducts tests aimed at the laboratory diagnosis of parasitic infections. To this end, direct microscopic imaging and permanent staining are used to test for protozoa and helminthes. Culture methods are also used to test for various protozoa. Stool samples arriving at the laboratory are microscopically examined using semi-automated systems and a diagnosis on a type basis can be made with the aid of stains such as trichrome and MAF. Diagnoses can also be made in relation to samples such as blood, sputum, bronchoalveolar lavage (BAL) and cyst fluid with the aid of florescent microscopy.

Our Molecular Microbiology unit conducts tests that allow us to identify disease-inducing microorganisms in clinical samples at the DNA and RNA level. This involves the use of PCR, real-time PCR, strip hybridization and DNA strain analysis using the latest, most secure methods once the nucleic acid is isolated via automated systems. In addition to their diagnostic use, these methods are also useful in monitoring the progress of treatments. Important microorganisms, particularly HBV, HCV, HIV and CMV, can be quantitatively tested for in clinical samples, and the reduction or increase in virus growth can help clinicians in determining whether the treatment being applied is effective. Over thirty microorganisms in differing clinical samples can be tested for at our Molecular Microbiology unit.